Bioethanol production involving recombinant C. thermocellum hydrolytic hemicellulase and fermentative microbes.
Identifieur interne : 002B80 ( Main/Exploration ); précédent : 002B79; suivant : 002B81Bioethanol production involving recombinant C. thermocellum hydrolytic hemicellulase and fermentative microbes.
Auteurs : Saprativ P. Das [Inde] ; Rajeev Ravindran ; Shadab Ahmed ; Debasish Das ; Dinesh Goyal ; Carlos M G A. Fontes ; Arun GoyalSource :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2012.
Descripteurs français
- KwdFr :
- Amorces ADN (MeSH), Bioréacteurs (MeSH), Candida (métabolisme), Clonage moléculaire (MeSH), Clostridium thermocellum (enzymologie), Clostridium thermocellum (métabolisme), Fermentation (MeSH), Glycosidases (génétique), Glycosidases (métabolisme), Hydrolyse (MeSH), Protéines recombinantes (métabolisme), Réaction de polymérisation en chaîne (MeSH), Séquence nucléotidique (MeSH), Électrophorèse sur gel de polyacrylamide (MeSH), Éthanol (métabolisme).
- MESH :
- enzymologie : Clostridium thermocellum.
- génétique : Glycosidases.
- métabolisme : Candida, Clostridium thermocellum, Glycosidases, Protéines recombinantes, Éthanol.
- Amorces ADN, Bioréacteurs, Clonage moléculaire, Fermentation, Hydrolyse, Réaction de polymérisation en chaîne, Séquence nucléotidique, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Bioreactors (MeSH), Candida (metabolism), Cloning, Molecular (MeSH), Clostridium thermocellum (enzymology), Clostridium thermocellum (metabolism), DNA Primers (MeSH), Electrophoresis, Polyacrylamide Gel (MeSH), Ethanol (metabolism), Fermentation (MeSH), Glycoside Hydrolases (genetics), Glycoside Hydrolases (metabolism), Hydrolysis (MeSH), Polymerase Chain Reaction (MeSH), Recombinant Proteins (metabolism).
- MESH :
- chemical , genetics : Glycoside Hydrolases.
- chemical , metabolism : Ethanol, Glycoside Hydrolases, Recombinant Proteins.
- chemical : DNA Primers.
- enzymology : Clostridium thermocellum.
- metabolism : Candida, Clostridium thermocellum.
- Base Sequence, Bioreactors, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Fermentation, Hydrolysis, Polymerase Chain Reaction.
Abstract
The enhancement of the biomass productivity of Escherichia coli cells harbouring the truncated 903 bp gene designated as glycoside hydrolase family 43 (GH43) from Clostridium thermocellum showing hemicellulase activity along with its further use in simultaneous saccharification and fermentation (SSF) process is described. (Phosphoric acid) H(3)PO(4)-acetone treatment and ammonia fibre expansion (AFEX) were the pretreatment strategies employed on the leafy biomass of mango, poplar, neem and asoka among various substrates owing to their high hemicellulose content. GH43 showed optimal activity at a temperature of 50 °C, pH 5.4 with stability over a pH range of 5.0-6.2. A 4-fold escalation in growth of the recombinant E. coli cells was observed when grown using repeated batch strategy in LB medium supplemented with glucose as co-substrate. Candida shehatae utilizing pentose sugars was employed for bioethanol production. AFEX pretreatment proved to be better over acid-acetone technique. The maximum ethanol concentration (1.44 g/L) was achieved for AFEX pretreated mango (1%, w/v) followed by poplar with an ethanol titre (1.32 g/L) in shake flask experiments. A 1.5-fold increase in ethanol titre (2.11 g/L) was achieved with mango (1%, w/v) in a SSF process using a table top 2-L bioreactor with 1 L working volume.
DOI: 10.1007/s12010-012-9618-7
PubMed: 22383050
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Das</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam</wicri:regionArea><wicri:noRegion>Assam</wicri:noRegion></affiliation></author><author><name sortKey="Ravindran, Rajeev" sort="Ravindran, Rajeev" uniqKey="Ravindran R" first="Rajeev" last="Ravindran">Rajeev Ravindran</name></author><author><name sortKey="Ahmed, Shadab" sort="Ahmed, Shadab" uniqKey="Ahmed S" first="Shadab" last="Ahmed">Shadab Ahmed</name></author><author><name sortKey="Das, Debasish" sort="Das, Debasish" uniqKey="Das D" first="Debasish" last="Das">Debasish Das</name></author><author><name sortKey="Goyal, Dinesh" sort="Goyal, Dinesh" uniqKey="Goyal D" first="Dinesh" last="Goyal">Dinesh Goyal</name></author><author><name sortKey="Fontes, Carlos M G A" sort="Fontes, Carlos M G A" uniqKey="Fontes C" first="Carlos M G A" last="Fontes">Carlos M G A. 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Das</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam</wicri:regionArea><wicri:noRegion>Assam</wicri:noRegion></affiliation></author><author><name sortKey="Ravindran, Rajeev" sort="Ravindran, Rajeev" uniqKey="Ravindran R" first="Rajeev" last="Ravindran">Rajeev Ravindran</name></author><author><name sortKey="Ahmed, Shadab" sort="Ahmed, Shadab" uniqKey="Ahmed S" first="Shadab" last="Ahmed">Shadab Ahmed</name></author><author><name sortKey="Das, Debasish" sort="Das, Debasish" uniqKey="Das D" first="Debasish" last="Das">Debasish Das</name></author><author><name sortKey="Goyal, Dinesh" sort="Goyal, Dinesh" uniqKey="Goyal D" first="Dinesh" last="Goyal">Dinesh Goyal</name></author><author><name sortKey="Fontes, Carlos M G A" sort="Fontes, Carlos M G A" uniqKey="Fontes C" first="Carlos M G A" last="Fontes">Carlos M G A. Fontes</name></author><author><name sortKey="Goyal, Arun" sort="Goyal, Arun" uniqKey="Goyal A" first="Arun" last="Goyal">Arun Goyal</name></author></analytic><series><title level="j">Applied biochemistry and biotechnology</title><idno type="eISSN">1559-0291</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Bioreactors (MeSH)</term><term>Candida (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>Clostridium thermocellum (enzymology)</term><term>Clostridium thermocellum (metabolism)</term><term>DNA Primers (MeSH)</term><term>Electrophoresis, Polyacrylamide Gel (MeSH)</term><term>Ethanol (metabolism)</term><term>Fermentation (MeSH)</term><term>Glycoside Hydrolases (genetics)</term><term>Glycoside Hydrolases (metabolism)</term><term>Hydrolysis (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Recombinant Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (MeSH)</term><term>Bioréacteurs (MeSH)</term><term>Candida (métabolisme)</term><term>Clonage moléculaire (MeSH)</term><term>Clostridium thermocellum (enzymologie)</term><term>Clostridium thermocellum (métabolisme)</term><term>Fermentation (MeSH)</term><term>Glycosidases (génétique)</term><term>Glycosidases (métabolisme)</term><term>Hydrolyse (MeSH)</term><term>Protéines recombinantes (métabolisme)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Électrophorèse sur gel de polyacrylamide (MeSH)</term><term>Éthanol (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glycoside Hydrolases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ethanol</term><term>Glycoside Hydrolases</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Clostridium thermocellum</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Clostridium thermocellum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycosidases</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Candida</term><term>Clostridium thermocellum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Candida</term><term>Clostridium thermocellum</term><term>Glycosidases</term><term>Protéines recombinantes</term><term>Éthanol</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Bioreactors</term><term>Cloning, Molecular</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Fermentation</term><term>Hydrolysis</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Bioréacteurs</term><term>Clonage moléculaire</term><term>Fermentation</term><term>Hydrolyse</term><term>Réaction de polymérisation en chaîne</term><term>Séquence nucléotidique</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The enhancement of the biomass productivity of Escherichia coli cells harbouring the truncated 903 bp gene designated as glycoside hydrolase family 43 (GH43) from Clostridium thermocellum showing hemicellulase activity along with its further use in simultaneous saccharification and fermentation (SSF) process is described. (Phosphoric acid) H(3)PO(4)-acetone treatment and ammonia fibre expansion (AFEX) were the pretreatment strategies employed on the leafy biomass of mango, poplar, neem and asoka among various substrates owing to their high hemicellulose content. GH43 showed optimal activity at a temperature of 50 °C, pH 5.4 with stability over a pH range of 5.0-6.2. A 4-fold escalation in growth of the recombinant E. coli cells was observed when grown using repeated batch strategy in LB medium supplemented with glucose as co-substrate. Candida shehatae utilizing pentose sugars was employed for bioethanol production. AFEX pretreatment proved to be better over acid-acetone technique. The maximum ethanol concentration (1.44 g/L) was achieved for AFEX pretreated mango (1%, w/v) followed by poplar with an ethanol titre (1.32 g/L) in shake flask experiments. A 1.5-fold increase in ethanol titre (2.11 g/L) was achieved with mango (1%, w/v) in a SSF process using a table top 2-L bioreactor with 1 L working volume.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22383050</PMID><DateCompleted><Year>2012</Year><Month>12</Month><Day>07</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1559-0291</ISSN><JournalIssue CitedMedium="Internet"><Volume>167</Volume><Issue>6</Issue><PubDate><Year>2012</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Applied biochemistry and biotechnology</Title><ISOAbbreviation>Appl Biochem Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Bioethanol production involving recombinant C. thermocellum hydrolytic hemicellulase and fermentative microbes.</ArticleTitle><Pagination><MedlinePgn>1475-88</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12010-012-9618-7</ELocationID><Abstract><AbstractText>The enhancement of the biomass productivity of Escherichia coli cells harbouring the truncated 903 bp gene designated as glycoside hydrolase family 43 (GH43) from Clostridium thermocellum showing hemicellulase activity along with its further use in simultaneous saccharification and fermentation (SSF) process is described. (Phosphoric acid) H(3)PO(4)-acetone treatment and ammonia fibre expansion (AFEX) were the pretreatment strategies employed on the leafy biomass of mango, poplar, neem and asoka among various substrates owing to their high hemicellulose content. GH43 showed optimal activity at a temperature of 50 °C, pH 5.4 with stability over a pH range of 5.0-6.2. A 4-fold escalation in growth of the recombinant E. coli cells was observed when grown using repeated batch strategy in LB medium supplemented with glucose as co-substrate. Candida shehatae utilizing pentose sugars was employed for bioethanol production. AFEX pretreatment proved to be better over acid-acetone technique. The maximum ethanol concentration (1.44 g/L) was achieved for AFEX pretreated mango (1%, w/v) followed by poplar with an ethanol titre (1.32 g/L) in shake flask experiments. A 1.5-fold increase in ethanol titre (2.11 g/L) was achieved with mango (1%, w/v) in a SSF process using a table top 2-L bioreactor with 1 L working volume.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Das</LastName><ForeName>Saprativ P</ForeName><Initials>SP</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ravindran</LastName><ForeName>Rajeev</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Ahmed</LastName><ForeName>Shadab</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Das</LastName><ForeName>Debasish</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Goyal</LastName><ForeName>Dinesh</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Fontes</LastName><ForeName>Carlos M G A</ForeName><Initials>CM</Initials></Author><Author ValidYN="Y"><LastName>Goyal</LastName><ForeName>Arun</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>03</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Biochem Biotechnol</MedlineTA><NlmUniqueID>8208561</NlmUniqueID><ISSNLinking>0273-2289</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D006026">Glycoside Hydrolases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="C023305">hemicellulase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019149" MajorTopicYN="N">Bioreactors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002175" MajorTopicYN="N">Candida</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D048013" MajorTopicYN="N">Clostridium thermocellum</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000431" MajorTopicYN="N">Ethanol</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="Y">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006026" MajorTopicYN="N">Glycoside Hydrolases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>11</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>02</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>3</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>3</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>12</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22383050</ArticleId><ArticleId IdType="doi">10.1007/s12010-012-9618-7</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Ahmed, Shadab" sort="Ahmed, Shadab" uniqKey="Ahmed S" first="Shadab" last="Ahmed">Shadab Ahmed</name><name sortKey="Das, Debasish" sort="Das, Debasish" uniqKey="Das D" first="Debasish" last="Das">Debasish Das</name><name sortKey="Fontes, Carlos M G A" sort="Fontes, Carlos M G A" uniqKey="Fontes C" first="Carlos M G A" last="Fontes">Carlos M G A. Fontes</name><name sortKey="Goyal, Arun" sort="Goyal, Arun" uniqKey="Goyal A" first="Arun" last="Goyal">Arun Goyal</name><name sortKey="Goyal, Dinesh" sort="Goyal, Dinesh" uniqKey="Goyal D" first="Dinesh" last="Goyal">Dinesh Goyal</name><name sortKey="Ravindran, Rajeev" sort="Ravindran, Rajeev" uniqKey="Ravindran R" first="Rajeev" last="Ravindran">Rajeev Ravindran</name></noCountry><country name="Inde"><noRegion><name sortKey="Das, Saprativ P" sort="Das, Saprativ P" uniqKey="Das S" first="Saprativ P" last="Das">Saprativ P. Das</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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